During a direct user authentication, this is the URL to Authenticate a user with (provided by your SAML 2.0 provider).
If your SAML 2.0 connection is to a SalesForce connected App, in the SAML Login Information you would copy the SP-Initiated Redirect Endpoint to this property.
For an OKTA SAML 2.0 application, this URL is found from either the initial definition or editing and is accessed from the Sign-On Tab:
The value is 1, Identity Provider Single Sign-On URL:
This is the value that is entered in the Relying party WS-Federation Passive protocol URL property